SUPPLEMENTARY INFORMATIONSelf-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavusMartín A. Palazzolo
1, María L. Mascotti
1,2 , Elizabeth S. Lewkowicz
3 and
Marcela Kurina-Sanz
1,11 Area de Química Orgánica, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, INTEQUI-CONICET, San Luis (5700), Argentina.
2 Present address: Laboratorio de Biología Molecular, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, IMIBIO- SL CONICET, San Luis (5700), Argentina.
3 Laboratorio de Biotransformaciones, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal (1876), Argentina.
INDEX
1. Microbial screening
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page 3
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2. Biotransformation analysis by GC-FID
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page 5
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3. NMR analysis of biotransformation products
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page 6
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3.1. Compound 1b
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page 7
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3.2. Compound 2b
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page 10
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3.3. Compound 4b
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page 13
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3.4. Compound 5b
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……………………………………………………………
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page 16
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3.5. Compound 5c
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page 19
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3.6. Compound 6b
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……………………………………………………………
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page 22
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3.7. Compound 7b
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……………………………………………………………
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page 25
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3.8. Compound 8b
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……………………………………………………………
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page 28
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1.Microbial screeningThe strains used for the microbial screening are listed in
Table SI1. Culture media composition is detailed in
Table SI2.
Microorganism
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Culture medium
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Temperature (°C)
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Erwinia amylovora CECT 222
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2
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30
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Erwinia carotovora CECT 225
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33
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26
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Erwinia carotovora CECT 314
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33
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26
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Erwinia chrysanthemi CECT 509
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33
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26
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Nocardia asteroides CECT 3051T
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1m
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30
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Nocardia corynebacterioides CECT 420T
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1m
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30
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Pseudomonas putida CECT 324
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2
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26
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Pseudomonas stuzeri CECT 930
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2
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26
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Pseudomonas syringae CECT 126
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1
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26
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Streptomyces badius CECT 3275
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118
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28
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Streptomyces baldaccii CECT 3249
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118
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28
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Streptomyces baldaccii CECT 3256
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118
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28
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Streptomyces blastmyceticus CECT 3257
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118
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28
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Streptomyces cattleya CECT 3278
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118
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28
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Streptomyces setonii CECT 3276
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118
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28
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Streptomyces fladiae CECT 3271
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118
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28
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Streptomyces flavogriseus CECT 3129
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118
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28
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Streptomyces griseostramineus CECT 3273
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118
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28
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Streptomyces griseus CECT 3116
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118
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28
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Streptomyces halstedii CECT 3328
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118
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28
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Streptomyces mobaraensis CECT 3263
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118
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28
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Streptomyces netropsis CECT 3248
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118
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28
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Streptomyces phaeochromogenes CECT 3308
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118
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28
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Streptomyces sp. CECT 3322
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118
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28
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Streptomyces sp. CECT 3145
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118
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28
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Aspergillus flavus UBA 294
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CZ
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28
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Aspergillus fumigatus UNRC 78
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CZ
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28
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Pycnoporus cinnabarinus UNRC 03
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CZ
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28
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Rhizopus sp. UBA 1089
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CZ
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28
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Rhodotorula mucilaginosa UBA 236
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YM
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28
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Saccharomyces cerevisiae#
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YM
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28
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# Isolated from commercial baker’s yeast.
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Table SI1. Strains used for the microbial screening.
Culture
medium
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Composition per 1000 mL distilled H2O
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1
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5 g beef extract; 10 g casein acid peptone; 5 g NaCl
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1m
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5 g beef extract; 10 g casein acid peptone; malt extract 10 g; 5 g NaCl
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2
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1 g beef extract; 2 g yeast extract; 5 g casein acid peptone; 5 g NaCl
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33
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10 g yeast extract; 20 g glucose; 5 g CaCO3
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118
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4 g yeast extract; 10 g malt extract; 4 g glucose; 2 g CaCO3
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CZ
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5 g yeast extract; 30 g sucrose; 1 g K2HPO3; 10 mL CZ concentrate#; 1 mL trace metal mix†
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YM
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3 g yeast extract; 5 g casein acid peptone; 10 g glucose; 2 g KH2PO4
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# CZ concentrate: 30 g NaNO3; 5 g KCl; 5 g MgSO4.7H2O; 0.1 g FeSO4.7H2O; 100 mL distilled H2O
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† Trace metal mix: 0.5 g CuSO4.5H2O; 1 g ZnSO4.7H2O; 100 mL distilled H2O
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